WebThe G and C bases have stronger hydrogen bonding and help with the stability of the primer. What is a good GC content for primers? The G-C content should be in the range of 30% to 80%, with 50% to 55% being ideal. If the primers G-C content is less than 50%, the length of the primer may need to be increased to maintain the proper Tm. WebNov 10, 2024 · Ensure that the FID temperature is high enough for ignition (>150 °C), the GC will not attempt to ignite the flame at a temperature <150 °C. You should set the FID temperature to 20 °C above the highest oven temperature, depending on the column type. Alternatively, you can use the Agilent recommended value of >300 °C, which provides a …
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